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spcas9 hf1 coding sequence  (Addgene inc)


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    Addgene inc spcas9 hf1 coding sequence
    Spcas9 Hf1 Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spcas9+hf1+coding+sequence/SpCas9-HF1+(Plasmid+%23138556)/pmc10025986-227-8-20
    Average 93 stars, based on 14 article reviews
    spcas9 hf1 coding sequence - by Bioz Stars, 2026-10
    93/100 stars

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    Construct:

    Article Title: Inhibition of adenovirus replication by CRISPR-Cas9-mediated targeting of the viral E1A gene
    Article Snippet: Vector designs and sequence analyses were performed in CLC Main Workbench 8.1.2. (QIAGEN, Hilden, Germany). .. To construct the individual Cas9/gRNA expression vectors, the SpCas9-HF1 coding sequence was amplified by PCR from plasmid VP12 (obtained from Addgene) with primers Cas9 HF1 FW and Cas9 HF1 RV, and the fragment was cloned into the Eco RI and Not I sites of pENTR4 (Thermo Fisher Scientific). .. The tetracycline-regulatable CMV-tetO2 promoter was amplified by PCR from pcDNA6.2-GW/EmGFP-miR-luc (Thermo Fisher Scientific) with primers CMV Tet02_FW and CMV Tet02_FW, and the resulting fragment was cloned into the Nco I and Eco RI sites of the same vector.

    Expressing:

    Article Title: Inhibition of adenovirus replication by CRISPR-Cas9-mediated targeting of the viral E1A gene
    Article Snippet: Vector designs and sequence analyses were performed in CLC Main Workbench 8.1.2. (QIAGEN, Hilden, Germany). .. To construct the individual Cas9/gRNA expression vectors, the SpCas9-HF1 coding sequence was amplified by PCR from plasmid VP12 (obtained from Addgene) with primers Cas9 HF1 FW and Cas9 HF1 RV, and the fragment was cloned into the Eco RI and Not I sites of pENTR4 (Thermo Fisher Scientific). .. The tetracycline-regulatable CMV-tetO2 promoter was amplified by PCR from pcDNA6.2-GW/EmGFP-miR-luc (Thermo Fisher Scientific) with primers CMV Tet02_FW and CMV Tet02_FW, and the resulting fragment was cloned into the Nco I and Eco RI sites of the same vector.

    Sequencing:

    Article Title: Inhibition of adenovirus replication by CRISPR-Cas9-mediated targeting of the viral E1A gene
    Article Snippet: Vector designs and sequence analyses were performed in CLC Main Workbench 8.1.2. (QIAGEN, Hilden, Germany). .. To construct the individual Cas9/gRNA expression vectors, the SpCas9-HF1 coding sequence was amplified by PCR from plasmid VP12 (obtained from Addgene) with primers Cas9 HF1 FW and Cas9 HF1 RV, and the fragment was cloned into the Eco RI and Not I sites of pENTR4 (Thermo Fisher Scientific). .. The tetracycline-regulatable CMV-tetO2 promoter was amplified by PCR from pcDNA6.2-GW/EmGFP-miR-luc (Thermo Fisher Scientific) with primers CMV Tet02_FW and CMV Tet02_FW, and the resulting fragment was cloned into the Nco I and Eco RI sites of the same vector.

    Amplification:

    Article Title: Inhibition of adenovirus replication by CRISPR-Cas9-mediated targeting of the viral E1A gene
    Article Snippet: Vector designs and sequence analyses were performed in CLC Main Workbench 8.1.2. (QIAGEN, Hilden, Germany). .. To construct the individual Cas9/gRNA expression vectors, the SpCas9-HF1 coding sequence was amplified by PCR from plasmid VP12 (obtained from Addgene) with primers Cas9 HF1 FW and Cas9 HF1 RV, and the fragment was cloned into the Eco RI and Not I sites of pENTR4 (Thermo Fisher Scientific). .. The tetracycline-regulatable CMV-tetO2 promoter was amplified by PCR from pcDNA6.2-GW/EmGFP-miR-luc (Thermo Fisher Scientific) with primers CMV Tet02_FW and CMV Tet02_FW, and the resulting fragment was cloned into the Nco I and Eco RI sites of the same vector.

    Polymerase Chain Reaction:

    Article Title: Inhibition of adenovirus replication by CRISPR-Cas9-mediated targeting of the viral E1A gene
    Article Snippet: Vector designs and sequence analyses were performed in CLC Main Workbench 8.1.2. (QIAGEN, Hilden, Germany). .. To construct the individual Cas9/gRNA expression vectors, the SpCas9-HF1 coding sequence was amplified by PCR from plasmid VP12 (obtained from Addgene) with primers Cas9 HF1 FW and Cas9 HF1 RV, and the fragment was cloned into the Eco RI and Not I sites of pENTR4 (Thermo Fisher Scientific). .. The tetracycline-regulatable CMV-tetO2 promoter was amplified by PCR from pcDNA6.2-GW/EmGFP-miR-luc (Thermo Fisher Scientific) with primers CMV Tet02_FW and CMV Tet02_FW, and the resulting fragment was cloned into the Nco I and Eco RI sites of the same vector.

    Plasmid Preparation:

    Article Title: Inhibition of adenovirus replication by CRISPR-Cas9-mediated targeting of the viral E1A gene
    Article Snippet: Vector designs and sequence analyses were performed in CLC Main Workbench 8.1.2. (QIAGEN, Hilden, Germany). .. To construct the individual Cas9/gRNA expression vectors, the SpCas9-HF1 coding sequence was amplified by PCR from plasmid VP12 (obtained from Addgene) with primers Cas9 HF1 FW and Cas9 HF1 RV, and the fragment was cloned into the Eco RI and Not I sites of pENTR4 (Thermo Fisher Scientific). .. The tetracycline-regulatable CMV-tetO2 promoter was amplified by PCR from pcDNA6.2-GW/EmGFP-miR-luc (Thermo Fisher Scientific) with primers CMV Tet02_FW and CMV Tet02_FW, and the resulting fragment was cloned into the Nco I and Eco RI sites of the same vector.

    Clone Assay:

    Article Title: Inhibition of adenovirus replication by CRISPR-Cas9-mediated targeting of the viral E1A gene
    Article Snippet: Vector designs and sequence analyses were performed in CLC Main Workbench 8.1.2. (QIAGEN, Hilden, Germany). .. To construct the individual Cas9/gRNA expression vectors, the SpCas9-HF1 coding sequence was amplified by PCR from plasmid VP12 (obtained from Addgene) with primers Cas9 HF1 FW and Cas9 HF1 RV, and the fragment was cloned into the Eco RI and Not I sites of pENTR4 (Thermo Fisher Scientific). .. The tetracycline-regulatable CMV-tetO2 promoter was amplified by PCR from pcDNA6.2-GW/EmGFP-miR-luc (Thermo Fisher Scientific) with primers CMV Tet02_FW and CMV Tet02_FW, and the resulting fragment was cloned into the Nco I and Eco RI sites of the same vector.



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